Publications

Andras Hutvagner, Dominic Scopelliti, Fiona Whelan, and Paul R Jaschke. (2021). Orthogonal translation using the non-canonical initiator-tRNA(AAC) alters protein sequence and stability in vivo. bioRxiv. DOI: 10.1101/2021.05.25.445580
Abstract

Ellina Trofimova and Paul R Jaschke. (2021). Plaque Size Tool: an automated plaque analysis tool for simplifying and standardising bacteriophage plaque morphology measurements. Virology. DOI: 10.1016/j.virol.2021.05.011
Abstract
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Github

Bradley W Wright, Mark P Molloy, and Paul R Jaschke. (2021). Overlapping genes in natural and engineered genomes. (Under Review).

Bradley W Wright, Dominic Y Logel, Mehdi Mirzai, Dana Pascovici, Mark P Molloy, and Paul R Jaschke. (2020). Proteomic and transcriptomic analysis of Microviridae φXI74 infection reveals broad up-regulation of host membrane damage and heat shock responses. mSystems. DOI: 10.1128/mSystems.00046-21
Abstract

Bradley W Wright, Juanfang Ruan, Mark P Molloy, Paul R Jaschke. (2020). Genome modularization reveals overlapped gene topology is necessary for efficient viral reproduction. ACS Synthetic Biology. DOI: 10.1021/acssynbio.0c00323
Abstract
Supplementary Information

Bradley W Wright, Juanfang Ruan, Mark P Molloy, Paul R Jaschke. (2020). Genome modularization reveals overlapped gene topology is necessary for efficient viral reproduction. bioRxiv. DOI: 10.1101/2020.06.10.143693
Abstract
Supplementary Information

Dominic Y Logel and Paul R Jaschke. (2020). A high-resolution map of bacteriophage ϕX174 transcription. Virology. 547: 47-56. DOI: 10.1016/j.virol.2020.05.008
Abstract

Paul R Jaschke, Gabrielle A Dotson, Kay S Hung, Diane Liu, Drew Endy. (2019). Definitive demonstration by synthesis of genome annotation completeness. Proceedings of the National Academy of Sciences of the United States of America. DOI: 10.1073/pnas.1905990116
Abstract PDF

Karen D. Weynberg and Paul R Jaschke. (2019). Building Better Bacteriophage with Biofoundries to Combat Antibiotic Resistant Bacteria. PHAGE: Therapy, Applications, Research. Accepted 30 Sept 2019. DOI: 10.1089/phage.2019.0005
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Paul R Jaschke. (2019). Simulated sandwich enzyme‐linked immunosorbent assay for a cost‐effective investigation of natural and engineered cellular signaling pathways. Biochemistry and Molecular Biology Education. 18 September 2019. DOI: https://doi.org/10.1002/bmb.21304.
Abstract PDF

Russel M. Vincent, Pandelitsa F. Yiasemides, Paul R Jaschke. (2019). An orthogonal amber initiator tRNA functions similarly across diverse Escherichia coli laboratory strains.
ScienceMatters. 1 May 2019. DOI: 10.19185/matters.201904000009
Abstract PDF

Russel M. Vincent, Bradley W. Wright, Paul R Jaschke. (2019). Measuring amber initiator tRNA orthogonality in a genomically recoded organism.
ACS Synthetic Biology. DOI: 2019. 10.1021/acssynbio.9b00021
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Paul R Jaschke, Gabrielle A Dotson, Kay Hung, Diane Liu, Drew Endy. (2018). Definitive demonstration by synthesis of genome annotation completeness. bioRxiv. 455428. https://doi.org/10.1101/455428

NAR BREAKTHROUGH ARTICLE
Ariel Hecht*, Jeff Glasgow*, Paul R. Jaschke*, Lukmaan Bawazer*, Matthew S. Munson, Jennifer Cochran, Drew Endy, Marc Salit. (2017). Measurements of translation initiation from all 64 codons in E. coli. Nucleic Acids Research. 45 (7): 3615-3626. DOI: 10.1093/nar/gkx070
*Authors contributed equally to the paper as first authors.

Figure 2. Translation initiation from all 64 codons. Normalized per-cell fluorescence measured from three replicate cultures, grown in LB and resuspended in PBS before measurement, with each of the 64 codons as the start codon in the GFP coding sequence. Shapes represent the replicate plate number, and filled shapes represent GFP expression significantly greater (adjusted P < 0.05) than the non-expressing control (Control) as determined by Dunnett’s test.

*Maxwell Bates, *Aaron Berliner, Joe Lachoff, Paul R. Jaschke, Eli Groban. (2016). Wet Lab Accelerator: A Web-Based Application Democratizing Laboratory Automation for Synthetic Biology. ACS Synthetic Biology. (2017), 6 (1), pp 167–171. DOI: 10.1021/acssynbio.6b00108
Abstract PDF
*Authors contributed equally to this work

Ariel Hecht*, Jeff Glasgow*, Paul R. Jaschke*, Lukmaan Bawazer*, Matthew S. Munson, Jennifer Cochran, Drew Endy, Marc Salit. (2016). Measurements of translation initiation from all 64 codons in E. coli. bioRxiv.
*Authors contributed equally to this work
Link

Hessel A, Quinn J, Jaschke PR. Sequence of Synthetic øX174 Bacteriophage. Museum of Modern Art (MoMA). Accessioned Item. New York, USA (Invited Exhibit)

Hessel A, Quinn J, Jaschke PR. Synthetic øX174 Bacteriophage. Design and Violence Exhibit. Museum of Modern Art (MoMA). New York, USA (Invited Exhibit).
Link

Jaschke PR, Ju Lu, Widya Mulyasasmita, and Luke J. Lee. (2013). Incyte Pharmaceuticals Is Primed For A Run. Seeking Alpha.
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Jaschke PR, Lieberman EK, Rodriguez J, Sierra A, Endy D. (2012). A fully decompressed synthetic bacteriophage øX174 genome assembled and archived in yeast. Virology. (Cover).
DOI: 10.1016/j.virol.2012.09.020.
Abstract PDF
Refactored øX174.1f genome sequence: JX913857

Our shared cover of a special bacteriophage issue of Virology.

Neupane B, Jaschke P, Saer R, Beatty JT, Reppert M, Jankowiak R. (2012). Electron Transfer in Rhodobacter sphaeroides Reaction Centers Containing Zn-Bacteriochlorophylls: A Hole Burning Study. The Journal of Physical Chemistry B. Mar 15; 116(10): 3457-3466. DOI: 10.1021/jp300304r
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Jaschke PR, Hardjasa A, Digby E, Hunter CN, Beatty JT. (2011). A bchD (Mg-chelatase) mutant of Rhodobacter sphaeroides synthesizes zinc-bacteriochlorophyll through a novel zinc-containing intermediates. The Journal of Biological Chemistry. Jun 10;286(23):20313-22. DOI: 10.1074/jbc.M110.212605
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Jaschke PR, Saer RG, Noll S, Beatty JT. (2011). Modification of the genome of Rhodobacter sphaeroides and construction of synthetic operons. Methods in Enzymology. 497:519-38. DOI: 10.1016/B978-0-12-385075-1.00023-8
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Jaschke PR. (2010). Discovery and characterization of a new zinc-bacteriochlorophyll biosynthetic pathway and photosystem in a magnesium-chelatase mutant. PhD Thesis. University of British Columbia. Abstract PDF

Jaschke PR, Drake I, Beatty JT. (2009). Modification of a French pressure cell to improve microbial cell disruption. Photosynthesis Research. 102(1): 95-7.
DOI: 10.1007/s11120-009-9493-4
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Our solution for French Presses that leak.

Lin S, Jaschke PR, Wang H, Paddock M, Tufts A, Allen JP, Rosell FI, Mauk GA, Woodbury NW, Beatty JT. (2009). Electron transfer in the Rhodobacter sphaeroides reaction center assembled with zinc bacteriochlorophyll. Proceedings of the National Academy of Sciences of the USA. 106(21): 8537-42.
DOI: 10.1073/pnas.0812719106
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Electron transfer in the Rhodobacter sphaeroides reaction center.

Jaschke PR, LeBlanc HN, Lang AS, Beatty JT. (2008). The PucC protein of Rhodobacter capsulatus mitigates an inhibitory effect of light-harvesting 2 alpha and beta proteins on light-harvesting complex 1. Photosynthesis Research. 95(2-3): 279-84. DOI: 10.1007/s11120-007-9258-x
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Jaschke PR, Beatty JT. (2007). The photosystem of Rhodobacter sphaeroides assembles with zinc bacteriochlorophyll in a bchD (magnesium chelatase) mutant. Biochemistry. 46(43): 12491-500.
DOI: 10.1021/bi701407k
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Table of Contents figure from our Biochemistry paper characterising a magnesium-chelatase mutant

Loiselle FB, Jaschke P, Casey JR. (2003). Structural and functional characterization of the human NBC3 sodium/bicarbonate co-transporter carboxyl-terminal cytoplasmic domain. Molecular Membrane Biology. 20(4): 307-17. DOI: 10.1080/0968768031000122520
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